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As a negative control, we performed RT-PCR using the same template without adding reverse transcriptase.
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A Ct value of 9.26 was obtained for conventional PCR, 23.29 for PD-PCR whereas 33.15 was observed in negative control (without adding template DNA) indicating amplification in both conventional PCR and PD-PCR reactions.
The single-crystalline LiMn2O4 nanorods were successfully synthesized by a simple, low-cost and eco-friendly approach in which the γ-MnOOH nanorods were prepared through a facile hydrothermal process, in which KMnO4 was reduced by anhydrous alcohol (CH3CH2OH) without adding any template reagent or additional surfactant.
As a control reaction, conventional PCR and PD-PCR were performed without adding any template DNA.
By using this alumina sol as precursor, an alumina material with uniform mesoporous distribution was formed after calcination at 550°C without adding any templates or other organic additives.
Metastable γ-Bi2O3 were successfully prepared via a controllable, facile and mild solution precipitation synthesis method in ethylene glycol-water mixed solvent systems without adding surfactant or template agents, at 80 ℃ and ambient atmospheric pressure in 40 min.
In this paper, we report a facile hydrothermal route for the shape-controlled synthesis of micro/nanostructured Bi2WO6, without adding surfactants or templates.
In particular, oral contraception is often prescribed as a six month supply without adding it to the repeat template.
Keep rubbing without adding water.
Coarse grind the dal without adding water.
Negative controls were run without added template.
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