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Amplification was carried out by using extracted genomic DNA as template with three chloroplast DNA (cpDNA) regions (trnL-F, rbcL and psbA-trnH 43,44,45. psbA-trnH 43,44,45
An ingenious unimolecular DNA template with three functional parts has been designed: 5′-fragment as the miR-21 recognition unit, middle fragment as the miR-21 analogue amplification unit, and 3′-fragment as the 8 17 DNAzyme production unit.
Plasmid copy number was determined by quantitative Real Time PCR, using three different concentrations of total genomic DNA as template, with three independent experiments for each concentration, using a 7300 Real-Time PCR System (Applied Biosystems, Foster City.
As a DNA template with three Sp1 binding sites, we used oligomer 15.
The RNA template with three Sp1 binding sites was synthesized from the PCR product primed with oligomers 1 and 2 (see above).
In this work, we designed a basic scaffoldin template with three divergent cohesin modules of different specificities and a CBM module, in which all of the modular components are separated by linker segments.
Similar(53)
Bosentan and its high-affinity analog K-8794 contain many aromatic moieties composed of a central pyrimidine template with four substituents: a 2-pyrimidyl, a 4-sulfonamide, a 5-phenol, and a 6-hydroxyl (Fig. 3a).
A custom template with six tissue classes was used for unified segmentation and normalization.
The open-composition can be defined by a template with two component types.
We slide the patch over the recognition template with four pixels forward to ensure a sufficient feature-level difference.
We slide the patch over the recognition template with four pixels forward to ensure enough feature-level difference.
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