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Step 3 involved the completion of the alternative SmPC template with specific product information.
The cDNA encoding the mature P. oxalicum GZ-2 endo-1,4-β-mannanase gene was PCR amplified with PrimeSTAR™ HS DNA Polymerase (Takara, Dalian, China) using the recombinant plasmid pMD-man5A as a template, with specific primers manA-ef including an EcoRI site, and manA-er including an XbaI site.
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Meanwhile, templates with specific aspect ratio and packing manner are used to define the geometries of nanotubes.
Data obtained confirm the theoretical suggestions that templates with specific surface chemistry can be successfully used for the control of nucleation and morphology of protein crystals.
Biodegradable scaffolds are generally considered as indispensable elements for engineering living tissues as they are used as temporary templates with specific mechanical and biological properties similar to native extracellular matrix (ECM).
Gene-specific plasmids served as control templates with specific oligonucleotide primer pairs.
In environmental samples, PCR amplification of sample DNA templates with specific biomarkers specific primers and subsequent phylogenetic analysis of the amplified DNA products is the method of choice to detect any previously unknown microorganisms (Amann et al. 1995).
In frame of this model, SiO2 nanopowder can be considered as morphological template with high specific surface area that provides high concentration of carbon-related emission centers located on the silica surface.
For each sample, gene expression PCR was carried out using 2 µl of cDNA template with sequence specific primers.
cDNA was used as a template with primers specific for 45S (forward, 5'-CGGGTTATTGCTGACAGACGC-3'; reverse, 5'-CAACCTCTCCAGCGACAGG-3'; and β actin (forward, TGG GTG TGA TGG TTG GCA TGG; reverse, TAA GAA AGA TGG CTG GAA GAG GG -3').
For real time PCR, 3μl of cDNA was used as template with gene specific primers (Table 7).
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