Sentence examples for template with proper from inspiring English sources

The plasmid of pCMA-HAOCT4-3′UTRmu encoding a mutation of HA-human OCT4, including 3′UTR (deleted the miR-34a binding site), was generated using pCMA-HAOCT4-3′UTR as a template with proper primers (Supplementary Table S2).

The plasmid of pCMA-HAOCT4 encoding HA-human OCT4 without 3′UTR or pCMA-HAOCT4-3′UTR encoding HA-human OCT4 with 3′UTR was generated by inserting a PCR product from human OCT4 cDNA plasmid (Addgene, Cambridge, MA, USA) as a template with proper primers (Supplementary Table S2) into the plasmid pCMV-HA (Clontech, Mountain View, CA, USA) digested with EcoR I and Not I.

The plasmid of psiCHECKOCT4-3′UTR encOCT4g orT4 3′UTR or psiCHECKOCT4-3′UTR-DM encoding a mutation of OCT4 3′UTR (deleted the miR-34a binding site) was constructed using plasmid pCMA-HAOCT4-3′UTR as a template for PCR with proper primers (Supplementary Table S2).

For the docking purpose, the ATP site within VEGFR-2 was defined as the ligand-binding site following the template of 1Y6B, and ellagic acid was docked into VEGFR-2 with proper parameter setting.

Wooden brushes with proper bristles.

Proper meals with proper table manners.

Roasts come with "proper gravy".

José is a proper bar with proper tapas and proper sherry – all good.

The paper ends with proper conclusions.

Three of the selected structures were used as templates for Vl (identity 68 92%), five of them as templates for Vh (identity 66 86%) and one structure was used as a template for proper orientation of variable domains in Fv.

With proper manual inspection, it should be possible to use a COMPOSITION and a number of ENTRY subclasses to represent the clinical statements of different natural classes, e.g. OBSERVATION and INSTRUCTION in a COSMIC template.