Your English writing platform
Discover LudwigExact(1)
In the reference dataset, the DN and SN are represented in the same template, with positive factor loadings associated with the DN and negative factor loadings associated with the SN.
Similar(59)
In all experiments, control PCRs lacking cDNA template were performed to confirm the absence of contamination, and primer performance was verified with positive control cDNA template from brain, skin, or a mixture of liver, heart, spleen and kidney tissue.
Negative controls were performed with water as template and positive controls were plasmids with cDNA sequences for mouse NTPDase1, rat NTPDase2, and human NTPDase3, NTPDase5 and NTPDase6.
To rank the genes, Affymetrix probe-set identifiers were converted to HUGO gene symbols using OntoTranslate [ 38], which were then ranked in order of strength of correlation with the template (most positive to most negative Spearman's correlation coefficient).
Inhibition controls consisted of sample DNA spiked with positive material, while contamination controls utilised no template reagent samples.
Each PCR procedure was performed with a blank control (without DNA template) and positive and negative controls.
All sample plates were run with positive controls (T47D breast cancer cell line RNA) and no-template negative controls.
As a negative control we used lysates with no template added, whereas positive control contained 1 pmol of purified recombinant canine Rab6A spiked into the respective cell-free lysate.
A negative control, with no template, and a positive inter-run control were included for each gene in each qPCR run.
Start with positive conversation.
Reinforce positive behavior with positive rewards.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com