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A Rep molecule may bind onto a template with P RB and drop from the template with P RD.
A Rep molecule may bind onto an RNA template with P RB and drop from the template with P RD.
A linear DNA may turn into a template (unfolding) with P LTT / F SFD, whereas a circular DNA may turn into a template with P CTT / F SFD.
A linear RNA may turn into a template (unfolding) with P LTT / FSFR, whereas a circular RNA may turn into a template with P CTT / F SFR.
A linear RNA may turn into a template (unfolding) with P LRTT, whereas a circular RNA may turn into a template with P CRTT.
The chromosome (as a circular RNA) may become a template with P CRTT (the probability of a circular RNA turning to a template), which should be higher than P LRTT (the probability of a linear RNA turning to a template), with which individual ribozymes (as linear RNAs) may become templates.
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To construct the secretion expression cassette without the SED1 anchoring region, amplification was performed using the plasmid pIL2GA-SS as a template with primers P-EG2 and EG2-T.
In the 3D domain predicted from PDB templates with a p value of 1.31e-02 100Y fell at the end of a loop, right before the beginning of a beta.
When the ribozymes evolved to a more efficient form with a more complicated structure, they may no longer be able to act as good templates (with lower P LRTT, e.g., from 0.2 to 0.01, as shown in Figure 4B, bottom-left panel), then the chromosome would have an opportunity to emerge (provided that it adopted a strategy of circularity and self-cleavage).
1, 3, 5-trimethylbenzene was used as an organic structural directing agent to enhance the pore size, pore volume, pore density and surface area of the resulting CuO hybrid templated with Pluronic P-123.
Nucleotides and oligomers aligned adjacently on the RNA template may be ligated to each other with P TL (template-directed ligation) in a non-enzymatic way.
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