Exact(1)
Bottom left: The five lines represent an example of the contents of the gray cubes in the top left template (with n = 800, and p = 5).
Similar(59)
Each semirandom sequence was based on particular real genome used as template (e.g., the human genome): It had the same size with the template genome, and it imitated N-bp composition of the template genome, with N ranging from 1 to 4. Thus, we constructed four semirandom genomes based on a single actual genome sequence.
Since there are M utilities with N template load profiles each, O MN) calculations have to be performed by the third party.
DNA templates, with the N-terminal truncations indicated by the residues that now became the first mifM-encoded residues, were used to direct in vitro translation with Bs hybrid PURE system in the presence of 35S-methionine.
We prepared transcription templates with an N-terminal FLAG-tag sequence using the 'split-primer' PCR method (Additional file 2).
Rejection criteria for an N-MUP train (and its associated S-MUP) included an N-MUP template with less than 51 individual contributions, a non-Gaussian MU interdischarge interval (IDI) histogram, a coefficient of variation greater than 0.3 for the IDI and a non-physiological or inconsistent firing rate [ 17].
Benefiting from the conductive CBC-N fiber template with hierarchical architectures and its coupling with uniformly dispersed NiCo2S4 nanoparticles, the CBC-N@NiCo2S4 composite exhibits high capacitance of 1078 F g−1 at 1 A g−1 and excellent capacity retention of 94.6% (918.5 F g−1 at 5 A g−1).
For the TOA-based approaches, we assume knowledge of the template s(n), and estimate τ i by cross-correlating the received signal with the clean template: τ ^ i = arg max τ i ∑ n = 0 N - 1 z i ( n ) s ( n - τ i ).
When the template was 3′- 1, N-ϵdG TACT-5′, dATP was prefereN-ϵdG TACT-5′porated, whereas with the template 3′-(1, N-ϵdG TACT-5′, both dATP and dATP wase incorpreferentially
DOI: http://dx.doi.org/10.7554/eLife.06562.018 The crystal structure of T7 DNAP (Doublie et al., 1998) shows interactions with two template-bases (N + 1 and N + 2) immediately downstream of the primer-end at positon N and ∼90o bend between N + 1 and N + 2 template-bases.
The efficiency of transcription was ∼10-fold less on the poly-nucleosomal template (poly-N) compared with that on the same amount of the equivalent nucleosome-free template (F) and similar, therefore, to the efficiency of transcription on the mono-nucleosomal template.
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