Sentence examples for template with gene from inspiring English sources

For real time PCR, 3μl of cDNA was used as template with gene specific primers (Table 7).

For additional clones, if RACE was required, 1 μg of total RNA was processed using the SMARTer RACE cDNA amplification kit (Clontech) and this cDNA was used as a template with gene specific primers (Additional file 7: Table S7) and the universal primer mix as per the manufacturer's protocol.

For cloning of FL targets from the same jack pine and lodgepole pine individuals that had been subjected to high throughput sequencing, 50 ng of jack pine RNA and 90 ng of lodgepole pine RNA were converted to cDNA using the Superscript III First-Strand Synthesis System (Invitrogen) and used as a template with gene specific primers (Additional file 7: Table S7).

PCR amplification was performed using the CYP2C BAC DNA as a template with gene-specific primers (5 pmol), 0.5 mM dNTPs, 2 mM MgCl2, and 1 unit of AmpliTaq Gold DNA polymerase (Applied Biosystems) in a total volume of 20 µl.

Normalization for cDNA quantity was performed for each template with an Elongation factor gene (At5g60390) as control gene using the relative standard curve method (delta CT) according to the Biorad instructions.

The cDNA obtained served as a template for PCR with gene specific primer sets.

Aliquots of the generated cDNA were used as template for PCR with gene specific primers.

PCR amplification of Tex14 using SA affected and control cDNA extracted from the testis or genomic DNA as a template was performed with gene specific primers.

Amplifying cDNA templates with a gene specific reverse primer and the general trans-spliced leader forward primer should produce products when the trans-spliced leader sequence is present at the start of the message and that particular gene is being transcribed.

First strand sense and antisense cDNAs were developed with strand-specific reverse transcript primers (Table S3) (SuperScript™ III First-Strand Synthesis System, Invitrogen, Carlsbad, CA, USA) from 5 11 day, 3 month, 8 month and >24-month bovine testis total RNA and used as templates for qPCR with gene specific primer sets (Table S3).

Total RNA from the platelets of 12 controls and 12 AMM patients were used as templates for PCR with gene specific primers.