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Subsequently, the double titanium osteotomy template with correct cut-angulation was constructed.
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This method involves four key steps: (i) the design of paired complementary 54-mer oligonucleotides with 18 bp overlaps, (ii) the utilisation of sequential OE-PCR to synthesise full-length genes, (iii) the cloning and sequencing of four positive T-clones of the synthesised genes and (iv) the resynthesis of target genes by OE-PCR with correct templates.
Figure 2(d) shows the enhanced prediction with the best aligned template of correct protein-DNA binding mode prediction.
This map was further processed to extract, according to a statistical criterion, a "thresholded template", showing what portion of the images was significantly correlated with correct discrimination.
Specifically, pCS2-TALEN-L, pCS2-TALEN-R and pSP6-2sNLS-spcas9 plasmids with correct insertions were linearized and recovered as corresponding templates.
If the primers are specific, amplicon with correct size should be observed only in case where cDNA was used as a DNA template.
[Updated with correct link].
Updated with correct founding location.
Update: now with correct video!
Edited with correct revenue figure.
Measure the main girl's head to get the correct circumference on a template and follow the template with the evergreen branches.
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