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We also developed the required chemistry to elaborate this template with additional substituents and have used this chemistry to prepare some initial compounds that show selective inhibition of anaplastic lymphoma kinase (ALK).
In Study 2, the procedural information for the experiments is much more similar between the Titles Template condition and the No Template condition in Study 2. Similar numbers of observations are recorded in the 'Step-by-step' section of the template, with additional observations recorded in the Discussion and Conclusion sections, as shown in Fig. 11.
With different updating methods for the weights, two detectors are proposed: Hard Decision for MSTR (HD-MSTR) detector and Soft Decision for MSTR (SD-MSTR) detector, where HD-MSTR obtains the template based only on the previous detected symbols, while SD-MSTR constructs the template with additional information from the decision variables.
Units of analysis were comprised of individual forms (e.g., consent form template) or a combination of forms (e.g., primary consent template with additional secondary template for genetic research), much as they would be presented to a potential research participant.
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Figure 3 shows the PDMS pillar arrays molded into the master template treated with FOTS, with additional surface treatment using toluene or hexane solvent.
For additional clones, if RACE was required, 1 μg of total RNA was processed using the SMARTer RACE cDNA amplification kit (Clontech) and this cDNA was used as a template with gene specific primers (Additional file 7: Table S7) and the universal primer mix as per the manufacturer's protocol.
For cloning of FL targets from the same jack pine and lodgepole pine individuals that had been subjected to high throughput sequencing, 50 ng of jack pine RNA and 90 ng of lodgepole pine RNA were converted to cDNA using the Superscript III First-Strand Synthesis System (Invitrogen) and used as a template with gene specific primers (Additional file 7: Table S7).
To differentiate map tasks from "normal ones (i.e., non-MapReduce tasks), the MapReduce VC layer modifies their templates by adding "(<mapreduce>)"tags with additional information such as job id and stage.
The scientists have attempted to explore the coumarin template for synthesizing novel AChE inhibitors with additional pharmacological activities including decrease in beta-amyloid (Aβ) deposition and beta-secretase inhibition that are also important for AD management.
The second PCR was carried out with the first PCR product as template with the primers listed in Additional file 4 at 95°C for 1 min, 55-60°C 55-60°Cin, and 72°C for 1 min (35 cycles).
A 560-bp region of the third exon of CG6234 was generated by PCR using genomic DNA as template with the primers indicated in Additional file 4 (Primers used for qPCR and conventional PCR reactions).
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