Preliminary experiments identified RNA template concentrations, which yielded a product within a linear range for both HPRT and the UCPs, under the standard conditions defined below, and PCR products were sequenced to ensure specificity.
By the chosen approach, 6T molecules are forced to align parallel to the p-6P template molecules, which yields highly polarized photoluminescence (PL -emission of both sPL -emission
The optimal cDNA template dilution used in real-time PCR reactions was chosen which yielded a cycle threshold (Ct) value after 25-30 cycles and was determined for each gene target individually; equivalent dilutions were used for a time course.
Which yielded nothing for Spurs.
Of the reactions which yielded only generic products, 91% gave positive identifications in an assay re-run, resulting in a failure rate of just ∼0.4% for reactions containing amplifiable template.
All RT-PCR products were sequenced except those obtained from the samples from Greece and Sweden, which yielded weak bands despite the considerable viral RNA titers in the original fecal samples (5.02 × 10 and 5.76 × 10 RNA copies/μL of template, respectively).
Also included was 1 pmol of an internal DNA primer, ASV 40-20, which yields a 40-nucleotide run-off product to control for template amount [ 1].
Take Wachovia, which yields 5%.
which yields inequality (2.4).
which yields that (2.32).
