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A template which did not contain recognizable CTCF sites served as a negative control (lanes 11 12).
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To rule out DNA contamination in the RNA preparations, the qRT-PCR controls were performed with RNA templates which did not show any amplification.
Newer NGS platforms (3G) utilise single molecule templates, which do not require PCR amplification.
Many logo sites have templates which don't cost much.
Since 2D reads contain information from both template and complement strands, a non-redundant dataset would consist of 2D reads plus any remaining template or complement reads which did not get turned into 2D reads.
Compared to ProFunc's Reverse Templates method which does not depend on such knowledge, ETA is just as sensitive (61%) but significantly more accurate (100% vs. 67%).
— which did not.
Which did he mean?
"Which did you prefer?
Which did you portray?
Which did we prefer?
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