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We speculate that Rad52 helps Rad51 initiate strand invasion from a blocked end by using its ssDNA annealing function to anneal the 3′ blocked strand to the template when the dsDNA of the template is transiently opened.
As mentioned in the Results section, the activity of the RdRp was reduced both when measured on (i) the MutG4 template where the G-rich sequence is mutated or (ii) on the WT template when the G4 is stabilized by the ligand or potassium.
Note once again that Stacy and co-workers recently achieved about 93% of nanowire length uniformity by pulsed electrodeposition in a well-ordered homemade AAO template when the deposition temperature was between 1°C and 4°C [15].
In summary, we have achieved length uniformity of template-assisted electrodeposited nanowires over 95%, even in highly disordered commercial AAO template, when the deposition temperature was lowered down to subzero centigrade degrees.
All three enzymes were found to be equally efficient for either template when the matched G-primer was used (Table S1, Figure S1, and Figure S2).
Despite the modular and tunable features of the surface template method, it is still difficult to scale up the method, due to the limited surface area of the template when the JNPs are manufactured.
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We design a simple mechanism to produce a collection to record those historical templates when the object appearance changed largely.
In addition to the increased performance of the protected templates when the secret key of a user is kept safe, another advantage of biometric hashing lies in the ease of revoking a transformed template by changing the associated secret key.
The term classification is generally applied to algorithms for the assignment of items to given templates when the templates themselves and their numbers are known.
This copy number is approximately the minimum required to accurately quantify a mutation present in 5% of templates when the output is not limited by the NGS read depth.
In contrast, Li et al. suggested that an optimized MSP assay was unable to detect methylated templates when the fraction of methylated fragments was < 6.2% in parallel assays [ 26].
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