The sensitivity of the assay ranged from 102 to 106 and from 105 to 109 RNA copy numbers for individual corona-/torovirus templates when tested, respectively, with and without an excess of RNA from human cells.
The rate of concordant SNP calls was lower when testing with less template DNA (97.8% for 17 ng/μl, 94.3% for 10 ng/μl, and 81.2% for 4 ng/μl, all assays in 5 μl sample volume).
Nault et al. [ 64] reported such an issue when testing T. tabaci for Wolbachia; however, negative results in repeated PCR assays using the same template DNA confirmed that the infection was the result of contamination.
When testing more specific hypotheses, other methods of classification (e.g., restricting the DI in space and time, template-matching, Mahalanobis distance) could be chosen.
When testing dilutions of mixed HPV/host DNA in replicate runs, we observed errors in quantifying E2 and E6 amplicons of 5 40%, with greatest error at the lowest DNA template concentration (3 ng/μl).
Use common sense when testing an article.
The usefulness of the SSR assays for diversity analysis and taxonomic discrimination was demonstrated by constructing a phylogeny based on SSR polymorphisms, and by the demonstration that most were also functional when tested with template from tomato, pepper and potato.
When tested on the PdG-containing templates, pol η and pol κ failed to carry out extension.
When tested on control poly-A RNA templates, the cDNA synthesis yields using these barcode-tailed oligo-dT primers are indistinguishable from standard oligo-dT primers.
When tested for RT-PCR amplification, one of these primers (C05-3′) amplified a 669-nucleotide fragment using dsRNA obtained from purified virus particles as a template.
