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Cobalt nitrate hexahydrate (Co(NO3 2·6H2O) as the source of cobalt, hexamethylenetetramine [HMT] (C6H12N4) as a precipitator, and sodium citrate dihydrate (C6H5Na3O7·2H2O) as a template were used for the preparation of Co3O4.
100 ng of cDNA template were used for each reaction.
In brief, a 25-µL reaction mix containing 0.5 µM of each forward and reverse primers and 10 µL of extracted RNA template were used for the RT-PCR.
Twenty nanograms of DNA template were used for WGA according to the protocol from manufactures.
Equivalent amounts of cDNA template were used for amplification of fragments of XI-1 XI-2 and XI-K mRNAs.
Equal amounts of mRNA template were used for complementary DNA synthesis using the High Capacity cDNA Kit (Applied Biosystems).
Similar(51)
In only four of these cases, though, had the artificial template been used for the repair.
Plaques were picked into 50 μL water and boiled for 10 min, and 2 μL of the boiled template was used for circularization PCR as described above.
This contrasts with the authors' observations in stem cells, in which the introduced nucleotide template was used for the repair process.
Then the designed template was used for a secondary template to synthesize metal, semiconductor, and polymer one-dimensional nanoarrays.
The page template is used for the detailed presentation and is denoted by the template:pageTemplate property.
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