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Standardised amounts (200 ng) of template were subsequently reverse transcribed using the TaqMan Reverse Transcription Reagents kit (Applied Biosystems) in 20 µl reactions following the recommendations of the manufacturer.
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In a subcutaneous rat model, this template was subsequently remodeled into mineralized bone tissue, including bone-marrow cavities.
The Al template is subsequently removed by acid etching, affording silicon nanoparticles wrapped by the highly conductive nitrogen-doped carbon.
RNA template was subsequently degraded in 20 mM EDTA and 50 mM NaOH.
The template was subsequently digested with DpnI.
Each DNA template was subsequently unzipped.
The atropine template was subsequently washed away from the MIP film with distilled water.
The mRNA synthesized from this template was subsequently amplified and mutated with Qβ replicase.
The genomic DNA template was subsequently quantified and diluted to 2 ng μL-1 before inclusion in PCR.
The folic acid template was subsequently removed with a mixed methanol acetic acid (9 1, v: v) solvent solution.
The first-round template was subsequently used in nested second-round PCRs (Taq DNA polymerase, Roche, Burgess Hill, UK).
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