Sentence examples for template were subjected from inspiring English sources

Transcripts generated using closed-circular single-stranded M13 virus as the DNA template were subjected to RNA-seq.

In addition, in all experiments, appropriate negative controls containing no template were subjected to the same procedure to exclude or detect any possible contamination.

Aliquots of 2 μL of cDNA template were subjected to Q-PCR using FAM-labelled scorpion primers (ATDBio, University of Southampton, UK).

Based on these quantifications, 300 molecules μL−1 per template were subjected to another round of 3D analysis, either separately or combined into a single reaction.

In all the experiments, appropriate negative controls containing no template were subjected to the same procedure to eliminate or to detect whether any possible DNA contamination present.

The same amount of template was subjected to 25 cycles of PCR amplification by using β-actin primers as an internal standard so that quantitation of mRNA levels could be corrected among the samples.

After ligation, the template was subjected to PCR amplification using a generic primer that recognizes the adaptor sequence.

This template was subjected to PCR and sequenced, and the amplicon was shown to be a faithful copy of the original template.

When the same RNA template was subjected to one-step RT-PCR using the N9-specific primers, the detection limit of the system was 1 PFU per tube).

To synthesize capped RNA, each template was subjected to in vitro transcription with the mMessage mMachine® SP6 kit (Life Technologies, California, USA).

For PCR reactions, cDNA templates were subjected to PCR with Taq polymerase using gene specific primers (for pol λ: GCC CAG CTC AGC TCA GAG GAT GAA and CGT CGG TAA GAG CCA CAA GCC ACA; for actin: β-actin mRNA primers from Ambion).