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Non-Template-Controls (NTC's) and samples with the cleaned RNA as template were run to exclude contaminations with gDNA.
To check for eventual contamination of the PCR reagents, negative controls containing the PCR-reaction mixture but without the DNA template were run during each PCR analysis.
In the first task (equilibrium discrimination) the three networks (R2, R3, template) were run starting from different initial conditions and their occupancy in the different regions of the phase space defined by the "equilibrium" positions of Protein1, Protein3 and Protein4 were examined.
Controls (no template) were run for all primer pairs.
Controls (no DNA template) were run to ensure there was no amplification of contaminating DNA.
All reactions were performed in triplicate, and negative controls (without template) were run for each master mix.
Similar(45)
A negative control without cDNA template was run simultaneously with every assay.
A reaction omitting the DNA template was run as a negative control.
A negative control without cDNA template was run with every assay.
A negative control (without template) was run in each reaction plate.
A negative control without cDNA template was run in every assay and measures were performed in duplicates.
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