Sentence examples for template were quantified from inspiring English sources

Copy numbers of the target template were quantified in peripheral blood and peritoneal fluid through generation of standard curves by serial dilution of plasmids for EpCAM and the housekeeping gene β-glucuronidase (GUS) (105 - 101 plasmid copies).

All experiments were done with three independent biological replicates and each template was quantified in technical duplicate.

Selected ligation products (chimeric DNA template) are quantified by a subsequent quantitative PCR step.

Normalized DNA template was quantified by real-time PCR as described previously (Ammar et al. 2009; Smith et al. 2009), diluted to 8pM, and clusters were generated on a single-read flow cell.

Next-generation sequencing: we used the Illumina HiSeq2000 sequencing platform as described below: Normalized DNA template was quantified by real-time PCR, diluted to 8 pM, and clusters were generated on a single-read flow cell.

The total RNA templates were quantified by spectrophotometry and subjected to 1.0% formaldehyde denatured agarose gel electrophoresis.

The transcripts of Bmras, the same as Bmrp49, in prepared cDNA templates were quantified on a real-time thermal cycler, LightCycler 480 Real-Time PCR System (Roche Diagnostics, Indianapolis, IN, USA).

The cDNA templates were quantified by the QuantiFast™ SYBR Green PCR kit according to the manufacturer's instructions.

It should be noted that an extended mispriming check is required for the 3C method since hybrid templates are quantified.

Eluated template libraries were quantified and quality checked using the Qubit dsDNA HS Assay kit (Invitrogen) and on an Agilent Bioanalyzer (Agilent).

For all reactions, these steps were accomplished using the Life Technologies ES module of the Ion OneTouch 2 System, and template-positive ISPs were quantified using the Guava EasyCyte 5 (Millipore Technologies), obtaining >90% enrichment efficiency for all reactions.