DNA fragments containing a CmR resistance marker and a I-SceI recognition site amplified with primer pairs JVO-0303/JVO-0304 and JVO-7448/JVO-11078 from the template plasmid pWRG100 were integrated into the chromosomal sroC and gltIp region, respectively, by lambda Red recombinase expressed from pKD46 (Datsenko & Wanner, 2000).
Secondly, 55 individual data regarding awake cortical resection of the eloquent cortices were integrated on the spatially normalized template.
These IRES sequence elements were integrated into the 5′ untranslated region of the DNA template, in order to initiate protein translation in a cap-independent manner.
A well-known example is the development of structured reporting templates to be integrated into radiology information systems [14].
Additionally, linear dsDNA templates might be integrated in the genome, causing potential deleterious effects.
Happy New Year: Custom layouts include a wide variety of fonts and unique images in each template; text can be integrated atop certain images (like the title of a book across its jacket) for additional customization.
The NAST algorithm [29] tries to align new sequences against a precalculated multiple alignment template, and has been integrated into many commonly used 16S rRNA analysis tools such as Mothur [11] and GreenGenes [31].
Recent studies demonstrate that understanding of intentions is also due in part to the automatic reactivation of pre-stored templates that have been integrated over time from one's own motor skills and life experiences, consistent with the perspective of embodied cognition [2], [3], [5] [7].
The circular template in our study was integrated with an antisense oligonucleotide aptamer sequence and drug loading sites.
The platform has access to 100,000+ templates and images and is integrated with Hootsuite, HubSpot, Buffer, and Yext.
