Sentence examples for template were extended from inspiring English sources

The leftover contigs were checked by BlastN versus the template, and two contigs that were not covered by any part of the template were extended by several rounds of assembly versus the 454 reads until they were closed.

In a second round of PCR the DNA template was extended with these regulatory DNA sequences supplied in the RTS Wheat Germ Linear Template Generation Set, His6-tag (Roche Applied Science).

Primers annealed to unmodified or modified DNA templates were extended in the presence of single dNTPs.

At the highest enzyme concentrations used (32 nM) 72% of control templates were extended to full-length product.

The M1dG lesion blocked incorporation of the initial nucleotide opposite the lesion, but 14% of templates were extended to full-length product.

Information requirements regarding plant 3D CAD models are established, and then ISO 15926 reference data (classes, attributes, and templates) are extended to cover information requirements.

For steady-state kinetic analysis, the primer-template was extended in the presence of 0.1 33 nM pol ι enzyme (or up to 400 nM enzyme for R96G) with increasing concentrations of individual dNTPs for 10 min, when the maximum amount of extension products was ≤20% of total DNA substrates.

Template-enriched beads were extended at the 3′ end in the presence of terminal transferase and 3′ bead linker.

Briefly, two allele-specific primers labeled with Cy3 (P1) or Cy5 (P2) and a third locus-specific primer (P3) with an address sequence were first hybridized to the template and allele-specific primers were extended to cross the SNP site to reach the locus-specific primer.

Analysis of the bypass products revealed that the nucleotide inserted opposite the lesion site was complementary to the nucleotide on the 5′ side of the PdG lesion of the template strand; subsequently, the primers were extended, generating products with either mismatches or deletions.

Internal communications were extended.