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New sugar amide-pyrrolidine derivatives possessing the furano form of the carbohydrate template were designed and developed as efficient and stereoselective organocatalysts for asymmetric Michael additions of ketones to nitroolefins at room temperature.
To generate recombinant plasmids that contained truncations or mutations in the 5' portions of SMReV S7 segment, PCR primers that used pcDNA3.1-S7 as a template were designed and appear in Additional file 1. PCR products were cut and ligated into pcDNA3.1 vector with corresponding enzymes.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com