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The CNT arrays in AAO template were combined on silicon, glass, and polyimide substrates with the assistant of epoxy resin as the adhesive, respectively.

For each candidate mixture composition, 4.8 nmol forward PCR primer and 4 nmol template were combined in 100 µL 1X KOD XL DNA Polymerase Buffer (EMD Chemicals), heated to 95°C for 8 minutes, and cooled on ice.

For real-time RT-PCR, 2  μg aliquots of RNA as template were combined with random hexamers and reverse transcriptase, according to the manufacturer's protocol (Invitrogen).

Each RT reaction was diluted 10 times in RNase-free water and 8 μl of the cDNA template was combined to the ExiLENT SYBRgreen master mix.

The template was combined with primer PT2F3 (5'- TCT CGG GAA GCG CGC CAT TGT-3') in an ABI 96-well Optical Reaction Plate (P/N 4306737) following the manufacturers recommendations.

Template was combined with 5 μl 10× PCR buffer (NEB), 1 μl 10 mM dNTPs, 1 μl each of 10 μM primer and 0.25 μl Taq (NEB) and ddH2O to a final volume of 50 μl.

The transformations to the FDG-PET-template were combined with the transformation to the original-space T1-MRI and the study-specific template.

cDNA templates were combined with SYBR Green premix with Rox (Takara) to perform quantitative-PCR reactions.

When the three control templates were combined, this effect led to a striking multimodal distribution in the fluorescence amplitudes (Fig. 3A).

Gene-specific primers at a final concentration of 200 nM and 1 μl of cDNA templates were combined into 20 μl reaction mixes and run through 40 cycles of PCR.

Both templates are combined with concatenation based feature level fusion technique and generate a combined template.