Sentence examples for template were carried from inspiring English sources

Additionally, semi-nested PCR reactions using 20F/1492R and 20F/1406R amplifications as template were carried out similar to bacterial 16S rDNA reactions, with 30 cycles instead of 35.

qPCR reactions for each template were carried out in duplicate or triplicate in 96-well plates.

qPCR reactions for each template were carried out in triplicate on 96-well plates.

PCR reactions with genomic DNA as a template were carried out as previously described [ 40- 42] using a gradient PCR thermal cycler (Mastercycler Gradient; Eppendorf, Germany).

PCR amplification using 1 μl of the culture pellet as DNA template were carried out to select three positive colonies for each primer pair.

Cell-free viral RNA synthesis using 2 ng PB1-equivalent of vRNP as the enzyme source and an endogenous genomic vRNA template were carried out in the presence (lane 1) or absence (lanes 2 8) of 0.2 mM ApG.

A study of SAPO-11 synthesis using typical compositions of starting gel with low times of crystallization and gels with low proportion of template was carried out.

Gene correction with single-stranded oligodeoxynucleotide (ssODN) as repair template was carried out as previously described with some modifications (Peters et al., 2013).

The removal of the PS template was carried out, and a porous Ag film, with regularly distributed nanopores (Figure 3c), was available for chemical etching to obtain the SiNW arrays.

The growth of Co nanowires in the InP membrane template is carried out in an electrochemical cell with a three-electrode configuration: a Pt counter electrode and a Pt pseudo-reference electrode, which are both immersed in the Co electrolyte.

Reverse transcription of each DNase-treated RNA template was carried out with random primer and M-MLV Reverse Transcriptase (Promega).