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The resulting fragments and template were assembled using In-Fusion HD Enzyme Mix (Clontech Laboratories, California, USA) and the resulting plasmid was validated.
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The multi-channel template is assembled via a facile hydrothermal and freeze-drying method with Si as precursor.
A DNA template was assembled from two PCR products.
Our workflow template was assembled using only component-types; however, individual components can also be used to build a workflow template.
The sequences to be used in building the alignment template are assembled from the result of a k-mer sequence search on the reference MSA.
Polystyrene spheres (PS) were synthesized by an emulsifier-free emulsion polymerization technique and the PS colloidal crystal templates were assembled orderly on clean glass substrates by dip-drawing method from emulsion of PS.
The immobilized DNA templates were assembled into chromatin (0.8 1 histone octamer/DNA ratio [wt/wt]) by salt deposition as described previously [ 48].
Groucho also did not stably associate when the factors were bound to naked DNA, or when templates were assembled with tail-less histone H3 and H4 nucleosomes, or when assembled with highly acetylated chromatin [ 40].
Mutation template plasmids were assembled by mixing 5'- and 3'- mutation fragments (125 fmol each) with pHA1877, a linear fragment of pUC19 obtained by PCR (see Additional file 1: Information) (25 fmol), and a selection cassette encoding an antibiotic resistance gene (75 fmol).
To assess templated mononucleotide ligation, minimal substrates were assembled from sets of upstream, downstream and bridging oligonucleotides.
DNA templates for in vitro transcription were assembled by ligating overlapping oligonucleotides, which included the T7 promoter, into pUC18 vector.
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