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The DIG-labeled dNTPs incorporated in the template were bound to anti-DIG-POD antibody.
Figure 9a indicates that nearly all pores of the template were filled by Cu nanowires.
Control reactions lacking cDNA template were also included to check for reagent contamination.
Carbon nanoparticles, which act as hard template, were added under vigorous stirring.
The width and length of the quartz template were 0.49 and 2.34 mm, respectively.
100 ng of cDNA template were used for each reaction.
Reaction mixtures without cDNA template were utilized as negative controls.
Triplicate incubations without template were used as negative controls.
Negative controls without template were added each time.
Control reactions containing no reverse transcriptase and no cDNA template were also performed.
Standards, samples and negative controls (no template) were analysed in triplicate.
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