Using TcTIM 2,3, 5 8 as the template, we prepared mutant TcTIM 2,3, 5 8 19E, 20S, 21L, 23V, 24P (reverting the first five different amino acids in region 1 of TbTIM to those found in the sequence of TcTIM).
Using BS B as a template we prepared the cross-inactivated variants NBS BW and WBS BN which repeated the asymmetric combination of mutations in WB BN originally used to test the electron transfer between the two hemes bL.
As PCR templates, we prepared total DNA (MAC+MIC) from the sequenced inbred T. thermophila strain SB210 and two other MIC-homozygous inbred strains, CU428 and B2086.
Using the 'split-primer' PCR and the 37 cDNA clones as templates, we prepared the transcription templates by adding the sequences of the SP6 promoter, E01 enhancer region, and Biotin ligase site (Bls) to the 5'-end (Fig. 1a).
To quantify the densities of a minor capsid protein gene (orf7) from WOSol and a heat-shock protein 60 gene (groEL) [ 5] from wSol in the DNA templates, we prepared standard solutions for the real-time qPCR.
"As we now enter the post-election phase, I look forward to working with vice-president-elect Pence and the rest of the leadership team to implement that template as we prepare for January 20 , 2017.
Using the template CNT, we prepared chemically stable, metallic nanoparticles with a well-defined size distribution and high rates of filling.
To further investigate the reliabilities of homologous peptide antigens derived from the template-based scoring function, we prepared 55 TCR-pMHC complexes as templates.
Alternatively, we prepared templates for the in vitro transcription of each truncated mRNA (without stop codon) at the 3′-end9,14 3′-end9,14
We prepared a template file for A. thaliana microarray ATH1.
