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Using equivalent amounts of starting total RNA template, we detected a miR-16 signal only with the RNA samples containing small RNAs (not shown).
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Although zβ1 variant C was identified by RACE-PCR using total embryonic RNA as template, we were unable to detect expression of this short splice variant in the adult tissues analyzed for this study.
5) The firing sequence templates used in the study are time templates, not spatial templates, detected via an algorithm based on cell-pair cross-correlation.
No DNA template was detected.
An equivalent ratio of wild-type to mutated template was detected using DNA and cDNA analysis.
However, in both of these cases no wild-type cDNA template was detected (case 4 and 7).
Consequently, only polymorphisms occurring at high frequency in one PCR template were detected as double peaks in the chromatograms.
We present here an analysis of colorectal cancer that supports our previous data published for glioblastoma, demonstrating that a higher amount of mutated P53 template is detected at the mRNA level than on the DNA level [ 1].
The precipitated polymer particles were collected by centrifugation and extensive batch-mode washing was performed by changing consecutively the washing solvent methanol-acetic acid (9 1) until no template was detected by HPLC.
Robetta also runs template-free modeling if a reliable template cannot be detected.
After 30 cycles of PCR, LDR of the Pfu-amplified template showed no tract length variation, but LDR of the Taq-amplified template detected tracts of 9, 10, and 11 guanosines (Additional file 2).
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