Because HR is a DSB repair system used during S-phase of the cell cycle, when sister chromatids are available as templates for DNA repair, we analyzed whether the induced DSBs observed in osrecql4 mutants could enhance HR.
We analyzed the origin of the donor templated loci and acceptor insertion loci.
We analyzed the expression patterns of the antibody fragment genes by semi-quantitative PCR from SMART cDNA template.
Using Nostoc punctiforme as member of the group with all traits analyzed as "template", we performed blastp analyses against all other genomes.
We analyze this effect in the context of macroporous polymer thin films formed from colloidal crystal templates.
LOH, loss of heterozygosity; MT, mutated template; MT>WT, prevalence of mutated template; NA, not analyzed; WT, wild type template; WT = MT, equal amount of wild type and mutated template; WT>MT, prevalence of wild type template; ROH, retention of heterozygosity; U, uninformative (samples 1 18, positive immunohistochemistry; samples 19 23, negative immunohistochemistry).
Briefly, we created a customized high-definition MR template, we converted SPECT scans to Analyze format using MRIcro (Columbia, SC, USA), and we coregistered them to their respective MR scans with SPM2 (SPM, Statistical Parametric Mapping, version 2; Functional Imaging Laboratory, London, UK).
Using thermal evaporation, we fabricated five uniform and regular arrays of Ag nanostructures with different shapes that were based on an anodized aluminum oxide template and analyzed their optical properties.
