Sentence examples for template we added from inspiring English sources

As template, we added 10 50 ng DNA to the reaction.

For amplification of BAC-templates we added 2 μl of overnight culture to the PCR, while amplification of genomic DNA was carried out by adding 2 μl DNA with a concentration of 45 ng/μl to the PCR.

To constrain transcription to a single round so that we can compare de novo PIC assembly on the two templates, we added 0.1% Sarkosyl immediately after the addition of nucleoside triphosphates (NTPs) for RNA synthesis following previously reported procedures using crude nuclear extracts (Hawley and Roeder, 1985; Kadonaga, 1990), which is also validated in our highly purified system.

By sequencing PCR 'blank' negative controls, specifically PCR-amplified ultrapure water with no template DNA added, we were able to distinguish between taxa that had originated from the DNA extraction kits as opposed to DNA from other sources (such as PCR kit reagents, laboratory consumables or laboratory personnel).

"We can do things around developer tools where we add JS templates, making your app use HTML5 app cache out of the box.

We used that as a template and added padding to shorts to create our own.

To that template Blondell added her own no-nonsense decency and warmth.

Carbon nanoparticles, which act as hard template, were added under vigorous stirring.

Two microliters of DNA template was added to 23 µL of LAMP mix, to a total volume of 25 µL.

Once a harmonic structure is recognized within Guard template at the current frame, these harmonic peaks will be extracted from Guard template and a corresponding new template is added to the original template matrix.

Negative controls without template were added each time.