Sentence examples for template water from inspiring English sources
The results showed that PCR products of the predicted size could be obtained using template cDNA, and the control without the template (water) gave no signals (Figure 5).
A pancreas (INS) or liver (IGF2) sample triplicate and a negative template (water) triplicate were included on each plate as a calibrator and negative control, respectively.
Control templates from human genomic lymphocyte DNA either treated with SssI methylase (methylated control) or untreated (unmethylated control) and a no template (water) control were included in each experiment.
Each PCR included six no-template (water) controls and a positive control, plasmid pXMRV, containing the full-length XMRV isolate, vp62, for which we are grateful to R. Silverman.
Negative controls consisting of no-template (water) reaction mixtures were run with all reactions.
No-template (water) reaction mixtures and no-RT mixtures were used on all samples as negative controls.
The plates for PCR each contained the genomic DNA samples, control DNA and a no-template water control in triplicate, and the experiments were performed in triplicate.
Template DNA and a non-template (water) negative control were hybridized with 384 different SNP loci containing OPA and an allele-specific multiplexed primer extension and ligation reaction was performed.
Controls included non-RT controls (using total RNA without reverse transcription to monitor for genomic DNA contamination) and non-template controls (water template).
Carbon microspheres as the sacrificial template and water as the hydrolysis agent were added to the solution.
The PCR reaction was set up in 50 μL reaction volume: 5 μL 10X pfu turbox buffer; 1 μL 10μM dNTP; 2.5 μL 10 uM forward and reverse primer; 1 μL pfu turbo cx polymerase (Agilent); appropriate amount of DNA template and water added up to 50 μL.
