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After successful re-amplification the template was utilized for probe generation.
A 0.5 μL of eluted template was utilized per each qPCR reaction in 20 μL final volume.
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For a single frame, in the spatial domain, a directional max-median filter is developed to make a pre-processing and a background suppression filtering template is utilized on the denoised image to highlight target.
Reaction mixtures without cDNA template were utilized as negative controls.
Retention of P-ends is expected when a sister chromatid template is utilized.
Copying by strand displacement produces two possible outcomes, depending upon which template is utilized.
The achieved silicon templates are utilized on the one hand for electrochemical deposition of a ferromagnetic metal and on the other hand to infiltrate nanoparticles.
Simple and facile sacrificial hard template strategy was utilized.
Similar template effect was utilized by pretexturing the aluminum surface to obtain different pore arrangement or different pore shapes since prepatterns will restrict the distribution of electric field [22, 23].
A capillary-driven particle-level templating technique was utilized to distribute graphite nanoplatelets (GNPs) into specially constructed architectures throughout a polystyrene matrix to form multi-functional composites with tailored electro-mechanical properties.
In this work, a solvent-free template synthesis technique was utilized to fabricate uniform arrays of substrate-bound poly(ɛ-caprolactone) (PCL) nanowires.
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