Sharing templates was previously available in Google Analytics, but as Google notes, being able to share the dashboard itself is a "nice complement" to this.
All cDNAs used as templates were previously normalized throughout β-actin RT PCR.
The opsin cDNAs with deduced amino acid sequences identical to those of the two alleles were created by the site-directed mutagenesis as in Hiramatsu et al. (2005) [13] using the squirrel monkey P560 opsin cDNAs as the template which was previously synthesized [62].
Plasmid pMK110 encoding the bgl transcription template was described previously (Haft et al., 2014).
We note that, because replication intermediates for 2D gel analysis were pretreated with RNaseA or RNaseH, gaps could also form at the lagging strand template where the RNA molecule was previously annealed.
The C- and G-rich telomeric DNA strand is always the leading- and lagging-strand replication template, respectively, and induced telomere dysfunction was previously observed to be strand specific in human cells (Bailey et al, 2001; Crabbe et al, 2004; Deng et al, 2009; Dimitrova & de Lange, 2009).
The C34 trimeric molecule was previously designed and synthesized using a novel template with C3-symmetric linkers by us.
To express MsrB1 in mammalian cells, the following constructs were generated: wild-type and cysteine (Cys) mutant cDNA of mouse MsrB1 (NM_013759) corresponding to Form 1 3'-UTR were amplified from pET28a MsrB1-Sec and pET28a MsrB1-Cys as templates that were previously constructed in the laboratory.
The DNA template was prepared as previously described [ 17].
The advantages of template stripping, which were previously demonstrated for Ag, Au, and Cu, are maintained: (i) the surfaces of the resulting films can be smooth without contamination, (ii) a variety of structures can be patterned, and (iii) integrated multilayer architectures can be created.
