Sentence examples for template was performed using from inspiring English sources

Real-time RT-PCR using synthesized cDNA as a template was performed using a Stratagene MX3000P qPCR system (Agilent/Stratagene, La Jolla, CA, USA) with four sets of PCR primers (BGL761(F) and BGL858(R), EGII968(F) and EGII1043(R), CBHII387(F) and CBHII455(R), and CDTI690(F) and CDTI761(R)) and Thunderbird SYBR qPCR Mix (Toyobo).

Conventional anodic aluminum oxide (AAO) template was performed using potentiostatic method of direct-current anodization (DCA) on costly high-purity (99.997%) aluminum foils at low temperatures of 0 10 °C to avoid dissolution effects which occurred frequently at room temperatures (RT) of 20 30 °C.

Amplification of 20 ng of DNA template was performed using specific primers.

The transcription factor binding site search using the −600 bp fragment of the Runx2 promoter as a template was performed using the Genomatix software suite (Munich, Germany).

The 5' to 3' elongation of the DNA sequence that produced a double-stranded DNA template was performed using Pwo DNA polymerase (Roche Diagnostics).

The standardization of cDNA template was performed using ubiquitin (UBC) primers [ 11].

Pairwise alignments between the primary amino acid sequence of each target and the respective structural template were performed using DeepView.

qRT-PCR reactions (20 μl volume containing 2 μl cDNA as the template) were performed using the StepOne real-time PCR system (Applied Biosystems 7500) in standard mode with the KAPA SYBR FAST Universal qRT- PCR kit (KAPA Biosystems).

First, hip templating was performed using one THA stem model, and it is not clear whether our conclusions apply for other commercially available femoral stems.

Real-time quantitative PCR of ChIP templates was performed using chromatin-region specific primers (Table S3) and Maxima™ SYBR Green/ROX qPCR Master Mix in a total volume of 10 µl in a LightCycler® 480 System.

Quantification of library DNA templates was performed using qPCR and a known-size reference standard.