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Decellularized liver tissue with a fine vascular tree network template was obtained using Triton X-100.
The AAO template was obtained by etching away the underlying aluminum substrates with a mercuric chloride solution.
After calcination at 550 °C for 6 h, the as-synthesized sample decomposed organic template was obtained.
A 10-nm-thick ZnO film was deposited by sputtering onto the GaN substrate as a seed layer, and the polymer hole template was obtained by nanoimprint lithography.
The grafting of PNIPAAm on the template was obtained by surface-initiated atom transfer radical polymerization (ATRP) leading to a reproducible and uniform brush film (15 nm thick) on the textured surface.
The unexposed regions were then removed using propylene glycol methyl ether acetate (PGMEA) (SU-8 developer, MicroChem) followed by rinsing with ethanol so that a 3D polymeric template was obtained [32 36].
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A well dispersion of amorphous MnO2 domains throughout carbon nanowalls template is obtained by potentiostatic anodic deposition technique.
An optimal amount for FW200 as hard template is obtained, which is said to be 10%% in weight.
The template is obtained by training some derivative curves reflecting different lips shapes except for closed lips.
Two mutants displaying 1.6- and 2.3-fold higher half-life τ1/2 °C5 °C) values than their DG9 template were obtained after screening only 384 clones.
A flexible disulfide-linked nanogel template is obtained by a controlled in-situ gelation method, enabling a high degree of control over each successive layer.
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