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This process was repeated until template was not detected by HPLC.
Further, in Region C (b), since the signal quality was deemed to be poor, hence the template was not updated.
This template was not included in our modeling protocol because of its significantly lower sequence similarity, and larger number of gaps (3%) when compared with the yeast template.
The template was then employed together with MRI during presurgical planning.[21] This procedure did give a proper location of eSF, however, the use of template was not an affordable method in all centers.
DNA synthesis along the ssDNA template was not critically affected when saturating amounts of NCp7 (1 NCperper 5 nucleotides) were added with increasing amounts of RT (from 6 nM to 500 nM), as shown after an electrophoresis of the deproteinized DNA products (Figure 3A).
Negative control samples were also run in which the template was not added.
Similar(41)
The template is not exact, since the case Wainwright reports is the modernisation (or "transformation", as it prefers to call it) of Newcastle council's IT services with 650 staff and a budget of £25m, not a national enterprise.
This thrill-free template is not universally popular but the Italian coach could win more hearts and minds if it proves to be the blueprint to avoid another European roasting from Real Madrid in a few weeks' time.
The template is not necessarily symmetric in our case.
Each project is unique and this template is not intended to cover unique, site-specific characteristics.
In fact, CLNF performs perfectly for closed lips for which the dynamic template is not suitable.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com