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The extraction of template was monitored by using HPLC.
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Stripping of the HATs from the templates was monitored using western blotting with an HA antibody (Covance) that recognizes the HA tagged Ada2 (for SAGA) or Esa1 (for NuA4) of these HAT complexes (data not shown).
Starting from a preordered SAM template, each step of the surface self-assembly was monitored by ToF-SIMS.
The incorporation by 1 µM NS5B was monitored on 1 µM 31-mer template (5'-CCAUAGAUAGCAUUGGUGCUCGAACAGUGAC-3') using GG as the priming sequence.
Amplification of 18S rRNA was monitored as an endogenous control that was used to normalize template amounts.
Acquisition of fluorescence signals was monitored on the iCycler and terminated, when all reactions reached an amplification plateau while a template-free control remained at basement level.
His breathing was monitored.
Development was monitored continuously.
Access resistance was monitored.
The fluorescence signals corresponding to the F57 sequence target and the IC template amplification were monitored during the 56°C annealing step in the LC Red 640 nm and LC Red 705 detection channels of the LightCycler 2.0 instrument, respectively.
However, assuming a linear trend, we can predict that for a sample size of 100 cells, at least 1 210 000 templates per gene to be monitored have to be present in the pre-amplification mixture for reliable measurements.
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