Sentence examples for template was initially from inspiring English sources

To improve construct validity, the template was initially tested on two pairs of units by two pairs of reviewers.

The chloride anion template was initially removed by washing with copious amounts of 0.1 m NH4PF6/H2O.

Before the amplification, the template was initially denatured by incubation at 94°C for 9 min then the amplification was performed for 35 cycles of 94°C for 30 s, corresponding annealing temperature for 30 s, and 72°C for 1 to 2 min depending on the amplified product.

Throughout a complex polymerization of glucose at 180°C in the autoclave, scalable pencil-like SnO2 nanorod arrays employed as the templates were initially coated by organic carbonaceous layers on their outer surface.

Templates were initially denatured at 95 °C for 30 s, and targets were amplified over 35 45 PCR cycles at 55-63 °C (Table  1).> CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega) was used for MTS assay as previously described [ 11] to measure cell proliferation.

DNA templates were initially denatured at 95°C for three minutes, followed by 30 cycles with denaturation at 95°C for 30 seconds, annealing at 60°C for 30 seconds and, extension at 72°C for 45 seconds and finally, an extension at 72°C for five minutes.

Template DNA was initially denatured at 94°C for 5 min prior to 35 cycles of denaturation at 94°C (30s), annealing at 55°C (30s), and extension at 72°C (45 s).

Template DNA was initially denatured at 94 °C for 2 mins followed by 35 cycles of PCR amplification with the following parameters: 30 s of denaturation at 94 °C, 40 s of primer annealing at 52 °C for RM23887, 55 °C for 21 and RM224, 58 °C for M265 and M355, 60 °C for M124 and NBS2-1, and 1 min of primer extension at 72 °C.

Template DNA was initially denatured at 94°C for 2 min followed by 35 cycles of PCR amplification with the following parameters: a 30 s of denaturation at 94°C, a 40 s of primer annealing at 55°C for markers 21, RM224 and PCR-Acc I, 65°C for maker sd1STS, and 1 min of primer extension at 72°C for markers RM224 and PCR-Acc I, and 1.5 min for markers 21 and sd1STS.

The perceptual template model was initially introduced in [50] and first applied to perceptual learning in [25], [51].

For each individual, the MNI template brain was initially registered into native fMRI space and the mean time course from a rectangular seed point (4 × 1 × 1 cm) was extracted.