Figure 16(b) on the other hand shows a different behavior when the number of templates is increased and the number of elements is kept constant (100 textual elements).
When the PCR result was unsatisfactory due to low DNA concentration, up to 0.5 μl DNA template was used; when DNA template volume was increased, dimethyl sulfoxide volume was reduced to maintain the total volume of 10 μl, accepting a slight shift in the overall ratio of ingredients.
The sensitivity of the assay might be higher if the amount of template DNA in the reaction was increased.
The conditions of PCR amplification were the same as those used in the DHPLC analysis except that genomic DNA rather than cDNA was used as template and the number of cycles was increased from 35 to 50.
However, when DNA from M184 or the three transformants was used as a template and the PCR extension time was increased to 10 min, a second PCR amplimer of approximately 9 kb was observed (http://epapers.bham.ac.uk/1958/).ac.uk/1958/
The KCl concentration was increased to 100 mM, and the primer-template annealed by slow cooling to room temperature.
The XRD pattern shows that the main phase is still anatase, however, when the template concentration is increased, minor peaks of the rutile phase appear and the crystallite size decreases to 8 nm.
Remarkably, the specificity constant for dCMP insertion across from template dG is increased over 400-fold when saturating concentrations of Mn2+ and Mg2+ are compared (Table 4).
Accordingly, the effective density of a free-stranding graphite foam grown on the high-surface area templates can be increased by an order of magnitude relative to one grown on a reticulated nickel foam template.
It was found that the average TM-score [31] of the first identified templates can be increased by 2.5% with the introduction of the torsion angle restraints, where the difference between ANGLOR-predicted angles and the experimental angles in templates was added to the profile-profile alignment scores.
