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Importantly, the resolution of barcode detection was nearly equivalent whether the starting template was from plasmid DNA or genomic DNA.
A single amplicon of expected size, 100 300 bp, with each primer set was observed on agarose gel electrophoresis, irrespective of whether the reverse-transcribed template was from AM roots or control roots.
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PCR-templates were from CNIO collection.
The secondary structures of templates are from DSSP [30].We have used the convention: (H, G, I) →H, (E, B) →E, and others →C.
The temporin-SHf template was identified from the antimicrobial peptide database (http://aps.unmc.edu/AP).unmc.edu/AP
The porous template was prepared from carbamide granules and then impregnated with epoxy resin.
The PCR fragments were separated by a 0.8% agarose gel and then a main PCR product of each template was extracted from the gel as described above.
This template was inspired from templates of Bertolino and Gnesi (2003), Cockburn (2000) and Gomaa (2004).
The SPECT template was aggregated from the first and second imaging of the test-retest animals.
The PS sphere template was removed from the substrate by ultrasonication in ethanol.
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