Sentence examples for template was done from inspiring English sources

The anchoring of Fe3O4 onto the graphene template was done by hydrothermal method involving polyvinylpyrollidone (PVP) as a surface directing agent.

Here, the deposition of metallic Zn nanowires, done to fill pores of the template, was done in two ways, by periodic and pulsed chemical electrodeposition.

Therefore, the creation of a MRI template was done by manual co-registration of all MR images based on prominent brain structures and the overall cortical contour and calculation of a mean image (Pmod 3.3, Pmod Technologies, Zurich, Switzerland).

Digestion of methylated template was done by 3 h incubation in 37°C with use of DpnI enzyme.

After the transcription reaction, removal of cDNA template was done by addition of RNase-free DNase (2 U) for 30 min at 37°C.

PCR reactions for each template were done in duplicate in 96-well plates.

PCR reactions for each template were done in triplicate in 96-well plates.

cDNA was amplified using the 5' PCR primer II A, and six 50 μl reactions (150 ng DNase-treated RNA per sample as template) were done using 21 PCR cycles.

Homology-based calculation of Colias PEPCK structures using these templates was done with MODELLER 9.8 [ 19]; in each case, 5 replicates were run and the best-scoring one (i.e. with lowest value of the molpdf criterion [ 19]) was used.

Only clear and reproducible marker bands were counted, and the comparison of bands between genomic and digested templates was done according to the appearance of the marker mentioned above.

Direct sequencing of PCR-generated templates was done using reactions based on the chemistry of BigDye Terminator v3.0 Cycle Sequencing Ready Reaction Kits (Applied Biosystems, Foster City, California, USA) with reactions set up in 96-well microtiter plates using a robotic workstation.