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A DNA template was assembled from two PCR products.
Our workflow template was assembled using only component-types; however, individual components can also be used to build a workflow template.
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The multi-channel template is assembled via a facile hydrothermal and freeze-drying method with Si as precursor.
The resulting fragments and template were assembled using In-Fusion HD Enzyme Mix (Clontech Laboratories, California, USA) and the resulting plasmid was validated.
The sequences to be used in building the alignment template are assembled from the result of a k-mer sequence search on the reference MSA.
Polystyrene spheres (PS) were synthesized by an emulsifier-free emulsion polymerization technique and the PS colloidal crystal templates were assembled orderly on clean glass substrates by dip-drawing method from emulsion of PS.
The immobilized DNA templates were assembled into chromatin (0.8 1 histone octamer/DNA ratio [wt/wt]) by salt deposition as described previously [ 48].
Groucho also did not stably associate when the factors were bound to naked DNA, or when templates were assembled with tail-less histone H3 and H4 nucleosomes, or when assembled with highly acetylated chromatin [ 40].
Template 7 was assembled from two fragments (A and B) by overlapping PCR.
Chromatin was assembled on the various DNA templates by exchange in high salt conditions with purified core particles (CP) from calf thymus (see [43] and references therein).
The molecule was assembled by using raloxifene as a template and substituting an indole ring for the benzothioprine core.
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