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Each template was analysed in triplicate.
Each sample of DNA template was analysed in triplicate for both Beta-globin and HPV16 DNA sequence for 40 cycles.
The nucleosomal composition of this template was analysed by MNase digestion, which produced a DNA ladder upon digestion of poly-nucleosomal DNA.
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Standards, samples and negative controls (no template) were analysed in triplicate.
Triplicate or quadruplicate samples of each template were analysed.
Sixty replicates of diluted template were analysed per sample.
All DNA templates were analysed in duplicates.
Proteins bound to the immobilized templates were analysed by SDS PAGE and detected by western blot using Pierce ECL kits.
In addition, ddH2O as the non-template control was analysed for every plate.
Flow cytometric data was analysed using a template previously created during optimisations in Flow Jo software (Treestar, Oregon, US) as described previously [15] and as illustrated in Fig. S1, with minor sample specific modifications between experiments.
Interview data was analysed using thematic template analysis to identify key themes surrounding student experience of podcast availability, particularly in relation to barriers to and facilitators of podcast use, and students' experiences of podcasts as a learning tool.
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