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A control tube with sterile water (Sigma) in place of template was always included.
A negative control reaction without template was always included for each primer combination.
A negative control reaction without a template was always included for each primer combination.
The specificity of PCR results was confirmed by melting curve analysis in which a negative control without a template was always included.
Because bisulfite-treated DNA is heavily degraded, the number of wells containing an amplifiable DNA template was always markedly lower than the number of DNA molecules in the starting sample.
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The template is always chosen prior to the spike.
Fourth, despite having eight additional mismatches to the target, wild-type repair templates were always more effective than their equivalent templates with I-SceI sites.
Templates are always contained within signs.
The Ct for all these "no-template" samples was always >45 cycles.
The ΔC t between samples and no template controls (NTCs) was always greater than five cycles, showing that contamination during the setup of the experiment was negligible.
"Whatever template we had, he was always on the earliest window, from when he started to throw to when he was off the mound to when he was in a rehab game," Angels Manager Mike Scioscia said.
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