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The template was again rinsed with deionized water.
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The resulting template is again transcribed by T7 RNA polymerase, creating a second amplification of aRNA.
The two clamp templates are again separated by a distance of 3 cm as was the case in the subtrochanteric region.
Using this plasmid as template, tetRA was again amplified using the primers T3 LR1R HindIII and T7 LR1F KpnI (Table S1) and cloned into pGEM-T Easy using TA cloning, creating the plasmid " tetRA-pGEM".
After that, the AAO template was treated again in a 5 wt% phosphoric acid solution at 35°C for 20 min to remove the AAO barrier layer.
After several PCR cycles of low-temperature annealing to allow the NLS primers to become incorporated into the DNA template, the annealing temperature is again increased.
Whose template was it?
And that template was here in London.
The template was secured by McGuigan.
My sexual template was still forming".
No DNA template was detected.
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