Sentence examples for template was added from inspiring English sources

Corresponding DNA template was added, and the experiment was conducted by following the manufacturer-recommended protocol.

Two microliters of DNA template was added to 23 µL of LAMP mix, to a total volume of 25 µL.

In brief, 2.4 g of cetyltrimethylammonium bromide (CTMABr) as the template was added to deionized water (120 g) and stirred to form a uniform solution.

The resultant mixture solution was continued to stir at room temperature for 10 min. The precursor solution was obtained after adding 0.4 g of hydrochloric acid (38%) to the P123/TEOS mixture solution and stirring at room temperature for 10 min. Then, the AAO template was added into the precursor solution at room temperature.

Briefly, 1 µl of cDNA template was added to the reaction mixture containing 10 µl of 2.5X Eppendorf mastermix (Eppendorf, Hamburg, Germany), 0.5 µM forward and reverse primers and nuclease free water to a final volume of 25 µl.

A total of 2 ul/well of template was added to the remaining sample wells along with Taqman Universal PCR master mix at a concentration of 1× and water to a volume of 25 ul/well.

Carbon nanoparticles, which act as hard template, were added under vigorous stirring.

Once a harmonic structure is recognized within Guard template at the current frame, these harmonic peaks will be extracted from Guard template and a corresponding new template is added to the original template matrix.

Negative controls without template were added each time.

Two oligonucleotide primers (0.2 µM each) and <1 µg of DNA template were added to the reaction mixtures.

For PCR amplification, in a 25 µl PCR reaction using DreamTaq Green PCR Master Mix (Fermentas, Burlington, Canada), 0.25 µl of forward and reverse primer (100 mM, Eurogentec, Liege, Belgium) and 0.5 µl cDNA template were added.