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To avoid potential template variation between assays, we used the same vial of Armored HIV RNA, heated to 70°C for 3 min to release the RNA from its bacteriophage-like complexes before cDNA synthesis.
If amplification failure is a measure of template variation, we find a much greater variability among plasmid-associated virulence factors.
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The recipe is useful as a template; variations will pop into your head – other vegetables, a spoonful of ricotta, a handful of capers to razz things up... Silver service is optional.
In the models, the secondary structure is similar to that of the template; variations only occur in loops, as often for modeled structures.
We discovered that template variations, including engineered terminal truncations, have significant influence on the position of the crossovers in the recombination-dependent PCR.
Individual miRNAs often exist as populations of variants (isomiRs) that differ in length and/or nucleotide composition generated by template- or non-template-directed variation (Burroughs et al., 2010; Newman et al., 2011; Neilsen et al., 2012).
However within the context of a species-specific song template, regional variation is extremely common.
The use of SYBR Green offers the advantage of real-time detection even in the case of template sequence variation, which may abolish detection using hybridization probes.
To characterize differences in the shape of the reaction norms among the different types (invasive vs. ancestral-range), we used a recently developed method called template mode variation (TMV) (Izem and Kingsolver 2005).
The range of error resulting from axial rotation compared with that from template marking variations is shown in Table 2. Given that rotational motion in the fixed segment model is 0°, and this error is affected more for anterior-posterior projections than for lateral ones, orthogonal radiographic positioning that minimises axial rotation is also important.
Further, photo- and soft lithography are used to generate master templates with variations in pattern shape, ridge width, and groove depth to influence cellular behavior and spatial outgrowth.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com