For real-time PCR 0.5 μg of cDNA or RNase-treated DNA was used as the template, utilizing Qiagen Quantitect Sybrgreen Mastermix on a Bio-Rad I-cycler (Hercules, CA).
Mesoporous NiCo2O4 hollow spheres are prepared by a soft template method utilizing a hydrothermal route.
We have previously reported the successful fabrication of electrodes on a bismuth nanowire encased in a quartz template by utilizing a combination of chemical mechanical polishing (CMP) and focused ion beam (FIB) processing.
The spirocyclic template utilized was designed on the basis of established architectures, and equipped with a mustard alkylating group.
This template utilizes the Sandia-developed QRA tool, Hydrogen Risk Analysis Model (HyRAM), to calculate risk values when developing risk-equivalent designs.
Soft template utilizes the intermolecular or intramolecular interaction forces, such as hydrogen bonds and bond and static electricity, to form aggregates with certain structural characteristics (liquid crystal, vesicles, micelle, microemulsion, self-assembled film, etc).
PCRs using genomic DNA as template utilized yeast lysed by microwave radiation as described previously [ 19] and.
The template matching utilizes a Euclidian distance as similarity measure.
Informed by the business strategy and utilizing a template containing learning from previous after action reviews, each stakeholder (e.g., marketing, engineering, design) creates a brief.
In their work, a suite of random loxP sites were generated by utilizing a template loxP oligonucleotide with degenerate spacers at positions 1,2,3,6,7,8.
