Sentence examples for template using random from inspiring English sources

Reverse transcription of the RNA was performed with the PrimeScriptTM 1st strand cDNA Synthesis Kit (TaKaRa) following the recommended protocol for synthesis of real-time PCR template using random primers.

First-strand cDNA was synthesized from 1 μg of total RNA template using random hexamers according to the high capacity cDNA archive kit (Applied Biosystems).

These were used as first-strand cDNA templates synthesised by using random hexamer primer.

For qRT-PCR analysis of Dictyostelium samples, total RNA was extracted from growing cells and used as template to generate cDNA using random primers (Roche 1st Strand synthesis kit).

Briefly, 1 µg of RNA template was reverse transcribed using random hexamer primers in buffered conditions (1× synthesis buffer; 0.1 M DTT, 40 U/µl RNaseOUT™; 15 U/µl Thermoscript™RT).

Two μg total RNA served as template for cDNA synthesis using random hexamers and the Omniscript cDNA Synthesis Kit (Qiagen).

The RNA was used as a template and reverse transcribed by using random hexamers and Superscript transcriptase (Life Technologies).

Using 1 µg of total RNA as template, single-stranded cDNAs were synthesized using random hexamers and TaqMan reverse transcription reagent kit (Applied Biosystems, Branchburg).

One µg of total RNA from each sample was treated with amplification grade DNase I and then used as a template for the reverse transcription reaction, using random hexamers and SuperScript II Reverse Transcriptase (Invitrogen).

With mRNA fragmentation as template, first strand cDNA was synthesized using random hexamer-primers and SuperScript II.

The purified mRNA was fragmented by the RNA fragmentation kit (Ambion) and applied as template for first-strand cDNA synthesis using random hexamer primers and reverse transcriptase (Invitrogen).