To speed-up, our method first aligns a target to all the templates using only the local alignment potential and then ranks all the templates using both the local and global alignment potentials described in Section 2. After ranking, only the first-ranked templates are used to build a 3D model by MODELLER for each target.
Our workflow template was assembled using only component-types; however, individual components can also be used to build a workflow template.
When the behavioral template was computed using only these trials, a diagnostic region emerged in the top part of the stimuli (see Fig. 9C, left) that was partially overlapping with the one obtained for the ideal observer (see Fig. 9C, right).
The accuracy of the models obtained is supported by: (i) the relatively high identity in amino acid sequences, (ii) the molecular mechanics calculations including explicit solvent with molecules of water and (iii) the use of several crystallographic templates for generating the hybrid models, which in this case have higher Z-scores than using only one template.
Furthermore, we estimate the upper bound of template-based binding site refinement using only weakly related proteins to be ∼2.6 Å RMSD.
In the second stage, PCR products from the first stage were used as the templates for a PCR reaction using only the flanking primers (F1, R2).
It can therefore represent a time-varying note by using only one single template.
An important strategy of the MALBAC method involves amplification using only the original template DNA, rather than exponential amplification, by protecting the amplification products (Fig. 3c).
Exclude "Template:" and use only the text thereafter.
For the recombinant gp120, this "copy paste" spectral pattern of glycopeptides facilitated identification of 2224 spectra using only 18 spectral templates, and after precursor mass correction, 1268 (57%) spectra were assigned to 460 unique glycopeptides accommodating 19 N-linked and one O-linked glycosylation sites (glycosites).
